Cationic cellulose nanocrystals for fast, efficient and selective heparin recovery

• Green and renewable cellulose nanocrystals were utilized for heparin recovery. Polycationic side chains grafted from nanocrystals. Instantaneous heparin-capture in biological conditions reduced recovery efforts. Selective heparin-recovery was achieved via salt concentration or pH adjustment. Significantly higher ability than Amberlite achieved. Heparin is one of the most important anticoagulant agents used clinical applications. Commercial production includes an isolation mucosa additional enrichment step by cationic resins. However, this process remains time-consuming while obtained very low concentrations with presence macromolecular impurities, such as proteins. Therefore, alternative a fast, efficient selective performance highly desirable. In work, we biomass-derived nanocrystal colloid conjugated polyelectrolytes The high specific surface area brush-like structure significantly increased speed efficiency under physiologically relevant conditions, which demonstrated methylene blue binding assay quartz crystal microbalance measurement. We also found that capture can be realized adjusting pH. Finally, showed after several recycle rounds, largely retained majority active dose recovered, showing commercial IRA-900 demonstrating its applicability economic perspective. reported nanocrystal-polymer conjugate represents promising candidate green